Intra-arterial infusion of human bone marrow-derived mesenchymal stem cells results in transient localization in the brain after cerebral ischemia in rats.
Identifieur interne : 000579 ( Main/Exploration ); précédent : 000578; suivant : 000580Intra-arterial infusion of human bone marrow-derived mesenchymal stem cells results in transient localization in the brain after cerebral ischemia in rats.
Auteurs : RBID : pubmed:23059455English descriptors
- KwdEn :
- Animals, Bone Marrow Transplantation (methods), Brain (cytology), Brain Ischemia (pathology), Cell Division (physiology), Cell Separation, Cells, Cultured, Flow Cytometry, Fluorescent Antibody Technique, Indirect, Infarction, Middle Cerebral Artery (pathology), Infusions, Intra-Arterial, Male, Mesenchymal Stem Cell Transplantation (methods), Organometallic Compounds (diagnostic use), Oxyquinoline (analogs & derivatives), Oxyquinoline (diagnostic use), Radiopharmaceuticals, Rats, Rats, Wistar, Tomography, Emission-Computed, Single-Photon.
- MESH :
- chemical , analogs & derivatives : Oxyquinoline.
- chemical , diagnostic use : Organometallic Compounds, Oxyquinoline.
- cytology : Brain.
- methods : Bone Marrow Transplantation, Mesenchymal Stem Cell Transplantation.
- pathology : Brain Ischemia, Infarction, Middle Cerebral Artery.
- physiology : Cell Division.
- Animals, Cell Separation, Cells, Cultured, Flow Cytometry, Fluorescent Antibody Technique, Indirect, Infusions, Intra-Arterial, Male, Radiopharmaceuticals, Rats, Rats, Wistar, Tomography, Emission-Computed, Single-Photon.
Abstract
Cell therapies from various sources have been under intense research in stroke. Efficient homing of the cells to the injured brain without complications is necessary to realize the therapeutic potential of cell therapy. Intra-arterial (IA) infusion of cells bypasses the filtering organs and directs the cells to the target area more efficiently. Here we studied the biodistribution of human bone marrow-derived mesenchymal stromal/stem cells (BMMSCs) after a direct infusion into the external carotid artery (ECA) in rats. Cells, which were cultured without animal-derived agents and also treated with a proteolytic enzyme to transiently modify cell surface adhesion proteins, were infused 24 h after transient middle cerebral artery occlusion (MCAO). SPECT imaging was used immediately after cell infusion and 24 h thereafter to track (111)In-oxine-labeled BMMSC in sham-operated and MCAO rats. IA infusion of BMMSCs in rats resulted in immediate cell entrapment in the brain, but the majority of the signal disappeared during the next 24 h and relocated to the internal organs. In MCAO rats, radioactivity counts 24 h after infusion were higher in the ischemic hemisphere compared to the contralateral hemisphere. Our results showed that IA infusion through ECA is a safe and efficient administration route for BMMSCs resulting in a transient localization of cells in the rat brain.
DOI: 10.1016/j.expneurol.2012.09.018
PubMed: 23059455
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Intra-arterial infusion of human bone marrow-derived mesenchymal stem cells results in transient localization in the brain after cerebral ischemia in rats.</title><author><name sortKey="Mitkari, Bhimashankar" uniqKey="Mitkari B">Bhimashankar Mitkari</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Clinical Medicine - Neurology, University of Eastern Finland, Kuopio, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Institute of Clinical Medicine - Neurology, University of Eastern Finland, Kuopio</wicri:regionArea></affiliation></author><author><name sortKey="Kerkel, Erja" uniqKey="Kerkel E">Erja Kerkelä</name></author><author><name sortKey="Nystedt, Johanna" uniqKey="Nystedt J">Johanna Nystedt</name></author><author><name sortKey="Korhonen, Matti" uniqKey="Korhonen M">Matti Korhonen</name></author><author><name sortKey="Mikkonen, Ville" uniqKey="Mikkonen V">Ville Mikkonen</name></author><author><name sortKey="Huhtala, Tuulia" uniqKey="Huhtala T">Tuulia Huhtala</name></author><author><name sortKey="Jolkkonen, Jukka" uniqKey="Jolkkonen J">Jukka Jolkkonen</name></author></titleStmt><publicationStmt><date when="2013">2013</date><idno type="doi">10.1016/j.expneurol.2012.09.018</idno><idno type="RBID">pubmed:23059455</idno><idno type="pmid">23059455</idno><idno type="wicri:Area/Main/Corpus">000994</idno><idno type="wicri:Area/Main/Curation">000994</idno><idno type="wicri:Area/Main/Exploration">000579</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Bone Marrow Transplantation (methods)</term><term>Brain (cytology)</term><term>Brain Ischemia (pathology)</term><term>Cell Division (physiology)</term><term>Cell Separation</term><term>Cells, Cultured</term><term>Flow Cytometry</term><term>Fluorescent Antibody Technique, Indirect</term><term>Infarction, Middle Cerebral Artery (pathology)</term><term>Infusions, Intra-Arterial</term><term>Male</term><term>Mesenchymal Stem Cell Transplantation (methods)</term><term>Organometallic Compounds (diagnostic use)</term><term>Oxyquinoline (analogs & derivatives)</term><term>Oxyquinoline (diagnostic use)</term><term>Radiopharmaceuticals</term><term>Rats</term><term>Rats, Wistar</term><term>Tomography, Emission-Computed, Single-Photon</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Oxyquinoline</term></keywords><keywords scheme="MESH" type="chemical" qualifier="diagnostic use" xml:lang="en"><term>Organometallic Compounds</term><term>Oxyquinoline</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Brain</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Bone Marrow Transplantation</term><term>Mesenchymal Stem Cell Transplantation</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Brain Ischemia</term><term>Infarction, Middle Cerebral Artery</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Division</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Separation</term><term>Cells, Cultured</term><term>Flow Cytometry</term><term>Fluorescent Antibody Technique, Indirect</term><term>Infusions, Intra-Arterial</term><term>Male</term><term>Radiopharmaceuticals</term><term>Rats</term><term>Rats, Wistar</term><term>Tomography, Emission-Computed, Single-Photon</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cell therapies from various sources have been under intense research in stroke. Efficient homing of the cells to the injured brain without complications is necessary to realize the therapeutic potential of cell therapy. Intra-arterial (IA) infusion of cells bypasses the filtering organs and directs the cells to the target area more efficiently. Here we studied the biodistribution of human bone marrow-derived mesenchymal stromal/stem cells (BMMSCs) after a direct infusion into the external carotid artery (ECA) in rats. Cells, which were cultured without animal-derived agents and also treated with a proteolytic enzyme to transiently modify cell surface adhesion proteins, were infused 24 h after transient middle cerebral artery occlusion (MCAO). SPECT imaging was used immediately after cell infusion and 24 h thereafter to track (111)In-oxine-labeled BMMSC in sham-operated and MCAO rats. IA infusion of BMMSCs in rats resulted in immediate cell entrapment in the brain, but the majority of the signal disappeared during the next 24 h and relocated to the internal organs. In MCAO rats, radioactivity counts 24 h after infusion were higher in the ischemic hemisphere compared to the contralateral hemisphere. Our results showed that IA infusion through ECA is a safe and efficient administration route for BMMSCs resulting in a transient localization of cells in the rat brain.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">23059455</PMID><DateCreated><Year>2013</Year><Month>01</Month><Day>01</Day></DateCreated><DateCompleted><Year>2013</Year><Month>02</Month><Day>28</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1090-2430</ISSN><JournalIssue CitedMedium="Internet"><Volume>239</Volume><PubDate><Year>2013</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Experimental neurology</Title><ISOAbbreviation>Exp. Neurol.</ISOAbbreviation></Journal><ArticleTitle>Intra-arterial infusion of human bone marrow-derived mesenchymal stem cells results in transient localization in the brain after cerebral ischemia in rats.</ArticleTitle><Pagination><MedlinePgn>158-62</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.expneurol.2012.09.018</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0014-4886(12)00380-9</ELocationID><Abstract><AbstractText>Cell therapies from various sources have been under intense research in stroke. Efficient homing of the cells to the injured brain without complications is necessary to realize the therapeutic potential of cell therapy. Intra-arterial (IA) infusion of cells bypasses the filtering organs and directs the cells to the target area more efficiently. Here we studied the biodistribution of human bone marrow-derived mesenchymal stromal/stem cells (BMMSCs) after a direct infusion into the external carotid artery (ECA) in rats. Cells, which were cultured without animal-derived agents and also treated with a proteolytic enzyme to transiently modify cell surface adhesion proteins, were infused 24 h after transient middle cerebral artery occlusion (MCAO). SPECT imaging was used immediately after cell infusion and 24 h thereafter to track (111)In-oxine-labeled BMMSC in sham-operated and MCAO rats. IA infusion of BMMSCs in rats resulted in immediate cell entrapment in the brain, but the majority of the signal disappeared during the next 24 h and relocated to the internal organs. In MCAO rats, radioactivity counts 24 h after infusion were higher in the ischemic hemisphere compared to the contralateral hemisphere. Our results showed that IA infusion through ECA is a safe and efficient administration route for BMMSCs resulting in a transient localization of cells in the rat brain.</AbstractText><CopyrightInformation>Copyright © 2012 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mitkari</LastName><ForeName>Bhimashankar</ForeName><Initials>B</Initials><Affiliation>Institute of Clinical Medicine - Neurology, University of Eastern Finland, Kuopio, Finland.</Affiliation></Author><Author ValidYN="Y"><LastName>Kerkelä</LastName><ForeName>Erja</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Nystedt</LastName><ForeName>Johanna</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Korhonen</LastName><ForeName>Matti</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Mikkonen</LastName><ForeName>Ville</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Huhtala</LastName><ForeName>Tuulia</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Jolkkonen</LastName><ForeName>Jukka</ForeName><Initials>J</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>10</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Exp Neurol</MedlineTA><NlmUniqueID>0370712</NlmUniqueID><ISSNLinking>0014-4886</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Organometallic Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Radiopharmaceuticals</NameOfSubstance></Chemical><Chemical><RegistryNumber>14514-42-2</RegistryNumber><NameOfSubstance>indium oxine</NameOfSubstance></Chemical><Chemical><RegistryNumber>5UTX5635HP</RegistryNumber><NameOfSubstance>Oxyquinoline</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Bone Marrow Transplantation</DescriptorName><QualifierName MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Brain</DescriptorName><QualifierName MajorTopicYN="Y">cytology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Brain Ischemia</DescriptorName><QualifierName MajorTopicYN="Y">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cell Division</DescriptorName><QualifierName MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cell Separation</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Flow Cytometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Fluorescent Antibody Technique, Indirect</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Infarction, Middle Cerebral Artery</DescriptorName><QualifierName MajorTopicYN="N">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Infusions, Intra-Arterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Mesenchymal Stem Cell Transplantation</DescriptorName><QualifierName MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Organometallic Compounds</DescriptorName><QualifierName MajorTopicYN="N">diagnostic use</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Oxyquinoline</DescriptorName><QualifierName MajorTopicYN="N">analogs & derivatives</QualifierName><QualifierName MajorTopicYN="N">diagnostic use</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Radiopharmaceuticals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Rats</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Rats, Wistar</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Tomography, Emission-Computed, Single-Photon</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>5</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2012</Year><Month>9</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>9</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2012</Year><Month>10</Month><Day>8</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>10</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>10</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>3</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pii">S0014-4886(12)00380-9</ArticleId><ArticleId IdType="doi">10.1016/j.expneurol.2012.09.018</ArticleId><ArticleId IdType="pubmed">23059455</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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